Agilent, 5190-2533, Agilent Bio SEC-5, 500Å, 4.6 x 300 mm, 5 µm

Agilent Bio SEC-5, 500Å, 4.6 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of large biological samples such as adeno associated virus. Resolving range 15 kDa - 5 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 15000 Da
Molecular Weight Upper Limit: 5000000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 500 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-5, 500Å, 4.6 x 300 mm, 5 µm is a high-performance size exclusion chromatography (SEC) column designed for the analysis and characterization of biomolecules, including proteins, antibodies, and synthetic polymers. With a 500 angstrom pore size and precisely engineered 5 micron particles, this column enables accurate molecular weight determination and effective separation of aggregates or fragments critical for biopharmaceutical development and quality control.

Optimized for aqueous SEC applications, it efficiently resolves proteins and other biological macromolecules under native conditions, preserving structural integrity during analysis. The robust silica-based matrix ensures high column stability, excellent reproducibility, and long operational lifetime, even with demanding sample loads.

The column is compatible with most standard HPLC and UHPLC systems, including those from Agilent, making it suitable for laboratories using Agilent 1100, 1200, or 1260 Infinity series instruments. It supports a wide range of mobile phases for flexibility in method development.

Ideal for biopharmaceutical research, quality control in therapeutic protein manufacturing, and academic studies, this column provides confidence in aggregate and oligomer detection. It integrates seamlessly with Agilent SEC calibration standards and is part of the comprehensive suite of Agilent Bio SEC columns, ensuring reliable and reproducible analytical performance.