Agilent, 884973-761, ZORBAX GF-250, 4.6 x 250 mm, 6 µm

ZORBAX GF-250, 4.6 x 250 mm, 6 µm. These legacy USP L35 zirconium-stabilized silica-based SEC columns have a pore size of 150 Å to separate proteins ranging from 4 to 400 kDa molecular weight.

Specifications:
Brand: ZORBAX
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 250 mm
Maximum Temperature: 60 °C
Molecular Weight Lower Limit: 4000 Da
Molecular Weight Upper Limit: 400000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 6 µm
Particle Type: Fully Porous
Phase: GF-250
Pore Size: 150 Å
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 3-8.5

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Designed for high-performance size exclusion chromatography (SEC), this high-quality column features a 4.6 x 250 mm dimension and a 6 micron particle size, making it ideal for precise molecular weight analysis and separation of proteins, peptides, and other biomolecules. With a robust silica-based matrix, it enables reliable resolution within the 1,000 to 250,000 Da molecular weight range, supporting critical biomolecular size distribution studies. Researchers benefit from its efficient flow properties and durable column packing, which provide reproducible results in both routine QC and advanced research applications.

This column is particularly suited for laboratories investigating recombinant protein purity, aggregate detection, or analyzing monoclonal antibody formulations. Commonly used in pharmaceutical, biotechnology, and academic research, it integrates seamlessly with Agilent HPLC systems and other compatible liquid chromatography platforms. Its design ensures minimized sample adsorption and high recovery rates, even for sensitive biomolecules. For extended performance or specific molecular weight ranges, alternative ZORBAX columns with different pore sizes and dimensions are available. Accessory guard columns and recommended mobile phases can further enhance its longevity and analytical throughput. Overall, this column supports robust, reproducible SEC analyses in demanding research environments.