Agilent, GKK-804, 2-AB-plus Labeling Kit

2-AB-plus Labeling Kit. Convenient fluorescent labeling of glycans with 2-AB (2-aminobenzamide) by reductive amination. Any purified glycan or glycan pool with a free reducing sugar may be labeled. The 2-AB-plus Labeling Kit improves on the traditional 2-AB Labeling Kit (p/n GKK-404) by providing the 2-AB and Reductant already in solution. You can now use what you need, and store the rest of the kit for later use, with no glass ampoules. GKK-804 can be used as a direct replacement for GKK-404: when mixed, the GKK-804 2-AB labeling solution will be the same as what is made when all components of GKK-404 are combined. Up to 48 samples may be processed.

Specifications:
Count: 48
Equipment & Reagents Provided by User: Acetonitrile, HPLC gradeCentrifugal evaporator (e.g., Savant, Heto or similar)GlykoPrep cleanup module (product code GS96-CU) or GlycoClean S cartridges (product code GKI-4726), 1 cartridge per sample for preand post-labeling cleanupHeating block, oven or similar dry heater set at 65°CReaction vials (e.g., polypropylene microcentrifuge vials)Water, HPLC grade
Technique: LC & LC/MS

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The 2-AB-plus Labeling Kit is a high-performance glycan labeling solution designed for reliable and sensitive fluorescent tagging of released glycans. Utilizing advanced 2-aminobenzamide chemistry, this kit enables efficient labeling of N- and O-linked glycans, facilitating downstream analysis by HPLC, UHPLC, or LC-MS. The streamlined protocol minimizes sample preparation time while ensuring high labeling efficiency and reproducibility, making it ideal for routine glycan profiling workflows in biopharmaceutical development, glycomics research, or quality control laboratories.

This kit is optimized to deliver strong and stable fluorescent signals, significantly enhancing detection sensitivity and quantitation accuracy for low-abundance glycan species. The provided reagents are sufficient for multiple reactions and are compatible with various sample types, including glycoproteins from biological or recombinant sources. Researchers benefit from its seamless integration with Agilent’s InfinityLab LC Series and 1260/1290 Infinity II LC systems as well as related glycan analysis columns and software packages.

Typical research applications include monoclonal antibody characterization, biosimilar comparison, and glycosylation pattern elucidation. The kit supports regulatory-compliant workflows and is adaptable for both manual and automated sample processing, providing flexibility for high-throughput laboratories or detailed structural glycan studies.