Agilent, GP24NG-AB, GlykoPrep Rapid N-glycan Prep with 2-AB, 24-ct

GlykoPrep Rapid N-glycan Prep with 2-AB, 24-ct. Complete kit is a cartridge based workflow with rapid digestion, labeling, and cleanup of excess 2-AB label. Includes GS24-RX digestion module, GS24-AB Reductive-Amination 2-AB labeling module, GS24-CU cleanup module.

Specifications:
Application: Compatible with microplate liquid handling on a broad range of automation platformsFlexible formatNonselective, rapid release and recovery of intact N-glycansPurified, fluorescently labeled N-glycans are produced in water and ready for analysis without concentrating or dryingRapid-reductive-amination labeling with 2-AB
Count: 24
Equipment & Reagents Provided by User: Acetonitrile (ACN, 100%, HPLC-grade)AssayMAP Labware (AM200)Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm heightFormic acid (HPLC-grade)Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)Ultrapure, deionized water
Kit Configuration: Sample Prep Kit
Label: 2-AB
Prep time (approx): 4 hours
Technique: LC & LC/MS

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This rapid N-glycan preparation kit streamlines the release and fluorescent labeling of N-glycans from glycoproteins, utilizing highly efficient 2-aminobenzamide (2-AB) derivatization. Optimized for quick, hands-on processing, the kit enables users to obtain ready-to-analyze N-glycans in under two hours, supporting high-throughput workflows in biopharmaceutical and glycomics labs. Each pack provides reagents and consumables for 24 individual preparations, minimizing sample loss and contamination.

The workflow is fully compatible with downstream analysis techniques, including HILIC-UPLC, HPLC, capillary electrophoresis, and mass spectrometry, ensuring robust glycan profiling and characterization for monoclonal antibody development, quality control, biosimilar comparability, and glycoprotein biomarker research. The 2-AB labeling allows for sensitive fluorescence detection and precise quantitation of released glycans.

This solution integrates seamlessly with Agilent's GlykoPrep analytical portfolios and is optimized for use with Agilent LC and CE systems as well as comparable instrumentation from other vendors. Users benefit from reliable, reproducible results across various sample types, from serum and plasma to purified recombinant proteins. GMP and regulated laboratory environments can adopt this kit for standardized, streamlined glycan analysis, complementing other Agilent glycan analysis reagents and columns.