Agilent, GP96NG-AB, GlykoPrep Rapid N-glycan Prep with 2-AB, 96-ct

GlykoPrep Rapid N-glycan Prep with 2-AB, 96-ct. Cartridge-based workflow with rapid digestion, labeling, and cleanup of excess 2-AB label. Complete kit includes GS96-RX digestion module, GS96-AB Reductive-Amination 2-AB labeling module; GS96-CU cleanup module

Specifications:
Application: Flexible, high-throughput format:process 1 to 192 samples per run (2 kits simultaneously)
Count: 96
Equipment & Reagents Provided by User: Acetonitrile (ACN, 100%, HPLC-grade)AssayMAP Labware (AM200)Centrifuge (capable of 50 - 1000 x g) and deep microplate rotor with clearance of minimum 44 mm heightFormic acid (HPLC-grade)Heater and incubation blocks, capable of 50–100°C, available from Agilent (GS150)Ultrapure, deionized water
Kit Configuration: Sample Prep Kit
Label: 2-AB
Prep time (approx): 4 hours
Technique: LC & LC/MS

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The GlykoPrep Rapid N-glycan Prep with 2-AB, 96-ct enables efficient, high-throughput preparation and fluorescent labeling of N-glycans from glycoprotein samples. Streamlining the glycan release and labeling workflow, this kit utilizes rapid enzymatic deglycosylation combined with 2-aminobenzamide (2-AB) tagging, yielding labeled glycans well suited for sensitive and quantitative detection. The pre-formulated reagents and plate-based format support simultaneous processing of up to 96 samples, making it ideal for large-scale studies requiring consistency and reproducibility.

Typical applications include glycan profiling for biotherapeutic development, quality control analytics, biomarker discovery, and fundamental research into protein glycosylation. The robust protocol is compatible with a wide range of sample types, from purified proteins to complex biological mixtures. Labeled glycans generated are directly amenable to downstream analysis using HPLC, UPLC, or capillary electrophoresis and offer compatibility with Agilent's AdvanceBio Glycan Mapping columns and related LC systems, as well as other major analytical platforms.

Designed to minimize hands-on time and maximize throughput, the kit supports efficient sample preparation workflows in pharmaceutical, biotechnology, and research laboratories seeking rapid, accurate glycan characterization.