Agilent, GS48-SAQ, AdvanceBio Total Sialic Acid Quantitation kit, 48-ct

AdvanceBio Total Sialic Acid Quantitation kit, 48-ct. Rapid quantitation of total sialic acid by fluorescence or absorbance detection, either free or released from intact glycoproteins. Automatable, 96-well microplate format with fluorescence detection. Broad range of detection, 40 pmol - 1,000 pmol. Sample digestion, conversion, detection and quantitation may be performed in a single well for fast and simple processing. Enzymatic cleavage allows rapid analysis (~75 minutes or less) with minimal, degradation of sialic acid.

Specifications:
Application: Automatable, 96-well microplate format with fluorescence detectionBroad range of detection, 40 pmol - 1,000 pmolEnzymatic cleavage allows rapid analysis (~75 minutes or less) with minimal, if any, degradation of sialic acidSample digestion, conversion, detection and quantitation may be performed in a single well for fast and simple processing
Count: 48
Equipment & Reagents Provided by User: Heat block with a flat surface to accept a 96-well skirted plate (e.g. VWR 13259-295 modular heating block for titer plate).Laboratory oven or block heater capable of 37°C incubation.Optional plate reader with 37°C temperature control (no need for the heater and block).Plate reader for measurement by fluorescence detection (530 nm excitation, 590 nm emission), or absorbance (530 nm).
Prep time (approx): ~75 minutes

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The AdvanceBio Total Sialic Acid Quantitation kit offers a comprehensive solution for accurate and efficient measurement of total sialic acid within glycoprotein and biologics research workflows. Designed for high-throughput laboratories, this kit supports up to 48 analyses and provides precise quantitation to facilitate quality control and characterization during biopharmaceutical development. The streamlined protocol allows for consistent sample preparation, hydrolysis, and sensitive quantification using fluorescence detection.

Suitable for a variety of sample matrices, this kit is ideal for applications including glycosylation profiling, biosimilar comparability studies, and lot-to-lot consistency verification. It enables researchers to monitor critical quality attributes that impact biological function and therapeutic efficacy of glycoproteins. The assay is compatible with Agilent liquid chromatography systems such as the 1260 Infinity II and 1290 Infinity II LC platforms, ensuring seamless integration into existing analytical workflows.

Paired with Agilent’s AdvanceBio Glycan Mapping kits and glycoprotein analysis columns, laboratories can broaden their glycan analysis capabilities for both routine and advanced glycomic studies. This complete solution empowers researchers with reliable data to support regulatory submissions and accelerate biologic drug development.