Agilent, GS96-SAQ, AdvanceBio Total Sialic Acid Quantitation kit, 96-ct

AdvanceBio Total Sialic Acid Quantitation kit, 96-ct. Rapid quantitation of total sialic acid by fluorescence or absorbance detection, either free or released from intact glycoproteins. Automatable, 96-well microplate format with fluorescence detection. Broad range of detection, 40 pmol - 1,000 pmol. Sample digestion, conversion, detection and quantitation may be performed in a single well for fast and simple processing. Enzymatic cleavage allows rapid analysis (~75 minutes or less) with minimal, degradation of sialic acid.

Specifications:
Application: Automatable, 96-well microplate format with fluorescence detectionBroad range of detection, 40 pmol - 1,000 pmolEnzymatic cleavage allows rapid analysis (~75 minutes or less) with minimal, if any, degradation of sialic acidSample digestion, conversion, detection and quantitation may be performed in a single well for fast and simple processing
Count: 96
Equipment & Reagents Provided by User: Heat block with a flat surface to accept a 96-well skirted plate (e.g. VWR 13259-295 modular heating block for titer plate).Laboratory oven or block heater capable of 37°C incubation.Optional plate reader with 37°C temperature control (no need for the heater and block).Plate reader for measurement by fluorescence detection (530 nm excitation, 590 nm emission), or absorbance (530 nm).
Prep time (approx): ~75 minutes

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
The AdvanceBio Total Sialic Acid Quantitation kit offers a robust and reliable solution for precise quantification of both free and total sialic acids in biopharmaceutical samples. With a 96-count format, it is designed to support high-throughput workflows in research and quality control environments. The kit streamlines sample preparation and detection, enabling accurate measurement of sialic acid content in glycoproteins, antibodies, and other biomolecules common in therapeutic development. Comprehensive, step-by-step protocols help ensure reproducibility and consistent results across replicates.

This quantitation kit is ideally utilized in the characterization of glycosylation profiles, supporting studies in bioprocess optimization, biosimilar comparability, and lot-to-lot consistency. Its sensitivity and specificity make it valuable in monitoring process changes and confirming compliance with regulatory requirements on glycosylation.

The kit is fully compatible with widely used Agilent LC and HPLC systems, maximizing workflow integration and reducing the need for specialized instrumentation. It may also be used alongside AdvanceBio Glycan Mapping kits for comprehensive glycan characterization. With ready-to-use reagents and straightforward procedures, this solution enables efficient, reliable analysis to support research and development demands in academic, biotechnology, and pharmaceutical laboratories.