Agilent, GX24-201PC, AdvanceBio Gly-X deglycosylation and InstantPC labeling module set, 24-ct

AdvanceBio Gly-X deglycosylation and InstantPC labeling module set, 24-ct. Utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantPC dye. With deglycosylation and labeling carried out in solution phase, the method is simple, rapid, and suitable for automation. The InstantPC dye delivers unmatched fluorescent brightness and MS signal, which enables a single labeling method to be deployed across different glycan analysis workflows. Includes GX24-100 Gly-X Deglycosylation Module, GX24-101 Gly-X InstantPC Labeling Module. For cleanup use GX96-102 Gly-X InstantPC cleanup module.

Specifications:
Application: Flexible, high-throughput format:process 1 to 24 samples
Count: 24
Deglycosylation Efficiency: Complete for most proteins. Protein load range: *More than 40 μg glycoprotein may be used, depending on the extent of glycosylation. %
Equipment & Reagents Provided by User: Note:Two GlykoPrep heaters, WS0271, can be fitted with VWR 13259-260 modular heating blocks
Kit Configuration: Sample Prep Module Set
Label: InstantPC
Precision (well-to-well, day-to-day): <5% CV
Prep time (approx): 42 min (16 samples), 99 min (96 samples)
Preservation of Sialylated Species: > 95 %
Protein Load Range: 1-40 µg
Recovery: > 95 %
Stability: Samples may be stored at -20°C for up to 6 months in Gly-X InstantPC eluent, or 4°C for up to 5 days in either Gly-X InstantPC eluent or after dilution with ACN/DMF
Technique: LC & LC/MS

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The AdvanceBio Gly-X deglycosylation and InstantPC labeling module set streamlines the preparation of N-glycans for high-sensitivity analysis in biopharmaceutical research and quality control. Featuring 24 pre-formulated sample sets, this module enables rapid, efficient enzymatic deglycosylation and fluorescent InstantPC labeling within a single workflow, minimizing manual intervention and sample handling time. Ideal for laboratories focused on monoclonal antibody glycoprofiling, biosimilar development, and therapeutic glycan characterization, the set delivers robust and reproducible results compatible with a range of analytical platforms. The labeled glycans produced by this workflow are specifically optimized for separation and quantification using LC-FLD, LC-MS, and CE-LIF systems, ensuring superior sensitivity and data quality. This module integrates seamlessly with Agilent high-performance LC systems, including the 1260 Infinity II and 1290 Infinity II series, and is complementary to Agilent AdvanceBio Glycan Mapping columns and MS detection workflows. By consolidating enzymatic digestion and labeling into a streamlined process, the module set accelerates sample turnaround and enhances reproducibility, supporting critical decision-making in bioprocess development and product characterization. This solution delivers confidence and efficiency for glycan analysis in regulated and research environments.