Agilent, GX96-401, AdvanceBio Gly-X 2-AB Express labeling module, 96-ct

AdvanceBio Gly-X 2-AB Express labeling module, 96-ct. Utilizes 2-AB (2-aminobenzamide) dye to derivatize released glycans. The proven 2-AB dye assures data continuity for ongoing projects.

Specifications:
Application: Flexible, high-throughput format:process 1 to 96 samples
Count: 96
Deglycosylation Efficiency: > 99 %
Equipment & Reagents Provided by User: Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended), ~4 mL per sampleDI water, ~100 µl per sampleGly-X vacuum manifold spacer and heater block lid (included in GX400 Gly-X 2-AB Express starter kit, or use GX100 spacer and alternate heater lid)Two Thermo Scientific 88871001 Compact dry block heaters with 96 well PCR heat blocks.Two dry block heaters with 96 well PCR heat blocks. One of the heaters can be substituted with a 96-well thermocycler. Denaturation and deglycosylation can be performed using a thermocycler. A heat block is required for the labeling step.Vacuum manifold (Millipore MSVMHTS00)Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220 V. Welch WOB-L Pump 2522)
Kit Configuration: Sample Prep Module
Label: 2-AB
Precision (well-to-well, day-to-day): <5% CV
Prep time (approx): 130 min (24 samples)
Preservation of Sialylated Species: > 99 %
Protein Load Range: 1-40 µg
Recovery: > 80 %
Stability: At least 1 month at -20°C, 5 days at 4–8°C
Technique: LC & LC/MS

**********The following product description is generated by AI and is for reference only. If you have any questions, please contact customer service.**********
Efficiently streamline N glycan sample preparation with this express labeling module designed for rapid, high throughput workflows. This module enables fast and reliable 2 aminobenzamide (2 AB) labeling of released glycans in a convenient 96 well plate format, making it ideal for users who require consistent, high quality results across multiple samples. It is optimized for use in biopharmaceutical development, glycoprotein characterization, biosimilar comparison, and quality control environments.

Compatible with automated liquid handling systems, this solution significantly reduces manual pipetting steps and sample handling errors while supporting efficient processing of complex sample batches. The provided procedure enables labeling within a short turnaround time, facilitating timely analysis and project advancement. Typical downstream applications include LC fluorescence and MS based glycan profiling. The labeled glycans are fully compatible with Agilent AdvanceBio Glycan Mapping columns and Agilent 1260 Infinity II LC systems, among others.

This module seamlessly integrates into existing glycan workflow solutions and is suitable for various sample types, including monoclonal antibodies and therapeutic glycoproteins. It aligns with regulatory expectations for structural characterization and batch consistency. As part of the AdvanceBio Gly X platform, it relates directly to other sample preparation and analysis modules, offering a streamlined end to end solution for glycan analysis.