Agilent, GX96-APTS, AdvanceBio Gly-X N-glycan prep with APTS Express kit, 96-ct

AdvanceBio Gly-X N-glycan prep with APTS Express kit, 96-ct. Complete kit utilizes a novel in-solution enzymatic protein deglycosylation followed by Express labeling of released N-glycans with 8-aminopyrene-1,3,6-trisulfonic acid (APTS). With deglycosylation carried out in solution, the method is simple, rapid and suitable for automation. After a simple cleanup step, the APTS-labeled glycan samples are ready for analysis by CE, CE/MS, and other methods. Includes GX96-100 Gly-X deglycosylation module (96-ct), GX96-601 Gly-X APTS Express labeling module (96-ct), GX96-602 Gly-X APTS Express cleanup module (96-ct).

Specifications:
Application: Flexible, high-throughput format:process 1 to 96 samples
Count: 96
Equipment & Reagents Provided by User: 96-well Thermocycler or two independent heat blocks, set for 90°C and 50°CAcetonitrile (ACN), MS-grade (Fisher A955-4 recommendedDI WaterThermocycler (Corning THERM-1001, 110 V. THERM-1000, 230 V)Vacuum manifold (Millipore MSVMHTS00-HTS)Vacuum manifold spacer (GX100, or GX400 Gly-X 2-AB/APTS starter kit is included free of charge with first Gly-X order, please contact us for details)Vacuum pump (Millipore WP6211560, 110 V. WP6122050, 220V. Welch WOB-L pump 2522)
Kit Configuration: Sample Prep Kit
Label: APTS
Prep time (approx): ~2.5 hours
Protein Load Range: 1-40 µg
Technique: CE

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The AdvanceBio Gly-X N-glycan prep with APTS Express kit, 96-ct is designed for fast and efficient fluorescent labeling and clean-up of N-linked glycans from biotherapeutic proteins. This comprehensive kit enables streamlined sample preparation with minimal hands-on time, supporting high-throughput analysis and robust, reproducible results. The included APTS labeling reagent allows for sensitive detection and quantitation via fluorescence, making the kit ideal for glycan profiling in both research and quality control environments.

Suitable for use with a variety of complex biological samples, the kit is optimized for workflows in biopharmaceutical development, biosimilar characterization, and biomarker discovery. The 96-count format supports large-scale screening and automation, promoting consistent glycan release and labeling for improved data comparability. This product is fully compatible with Agilent’s AdvanceBio LC columns, as well as a range of HPLC, UHPLC, CE, and MS analytical platforms commonly utilized in glycan analysis. Integration into existing AdvanceBio workflows offers streamlined method development and reproducibility. Related products, such as Gly-X clean-up cartridges and AdvanceBio Glycan columns, complement this kit for a complete glycan analysis solution. Researchers can confidently achieve detailed N-glycan profiles to accelerate discovery and development projects.