BD Pharmingen™ Biotin Mouse Anti-Human Toll-Like Receptor 4 551975

Alternative Name: TLR4; CD284; Toll-like Receptor 4; ARMD10
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Human TLR4-transfected Ba/F3 cell line
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 20 µl
RRID: AB_394311
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures The biotinylated HTA125 antibody can be used for the immunofluorescent staining along with Streptavidin-phycoerythrin (Cat. No. 554061) for flow cytometric analyses of human leukocytes and cell lines that express TLR4 (see Figure).
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.