BD, 558704, BD Pharmingen™ Dog Activated T Lymphocyte Cocktail

Reactivity: Dog (QC Testing)
Regulatory Status: RUO
RRID: AB_10429993
Description: Description The Dog Lymphocyte Activation Cocktail is a three-color reagent cocktail designed to identify dog activated lymphocytes by direct immunofluorescent staining with flow cytometric analysis.  The LSM 8.358 antibody reacts with a CD3-like T-cell receptor-associated cell-surface antigen found on thymocytes and peripheral T lymphocytes.  The LSM 11.425 antibody was generated against cells derived from canine peripheral lymph nodes and has been used as a prognostic tool in dog B cell lymphoma studies. The CTL 2.58 antibody was generated by using whole cell immunizations of IL-2 dependent feline T cell lines stimulated with PHA and Con A.  This clone reacts with dog and is referred to as the T Cell Activation Marker.
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
Recommended Assay Procedures: Recommended Assay Procedures BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.