BD, 560286, BD Phosflow™ Alexa Fluor® 647 Mouse anti-4EBP1 (pT36/pT45)

Alternative Name: 4E-BP1. EIF4EBP1, P/OKCL.6, PHAS-I, PHAS-1
Reactivity: Human (QC Testing), Mouse, Rat (Predicted)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Phosphorylated Human 4EBP1 (pT45) Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Vol. Per Test: 20 µl
RRID: AB_1645394
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Recommended Assay Procedures: Recommended Assay Procedures Either BD Cytofix™ fixation buffer or BD Phosflow™ Fix Buffer I may be used for cell fixation.  Any of the three BD Phosflow™ permeabilization buffers may be used.