Waters, 186002877, ACQUITY UPLC BEH C8 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1/pk

The ACQUITY BEH C8 sorbent is less retentive compared to the ACQUITY BEH C18 sorbent. A method developer may prefer the speed offered by a less retentive column, while improving peak shape and chromatographic performance. The trifunctionally bonded BEH particles offer the widest usable pH range (1-12), superior low pH stability, and ultra-low column bleed.

Specifications
Chemistry: C8
Separation Mode: Reversed Phase
Particle Substrate: Hybrid
pH Range Min: 1 pH
pH Range Max: 12 pH
Maximum Pressure: 18000 psi (1240 Bar)
Endcapped: Yes
Silanol Activity: Low
Particle Shape: Spherical
Particle Size: 1.7 µm
Endfitting Type: Parker-style
Pore Size: 130 Å
Format: Column
Surface Area: 185
System: UPLC, UHPLC
Particle Technology: BEH
USP Classification: L7
Inner Diameter: 2.1 mm
Length: 50 mm
Carbon Load: 13 %
eCord: Yes
UNSPSC: 41115709
Brand: ACQUITY UPLC
Product Type: Columns
Units per Package: 1 pk

Additional Information
ACQUITY UPLC BEH C8 Column, 130Å, 1.7 µm, 2.1 mm X 50 mm, 1/pk The ACQUITY UPLC BEH C8 columns have been designed to be suitable for most UPLC separations by providing the broadest range of pH stability (1-12). The ACQUITY UPLC BEH C8 columns, however, offer a distinct speed advantage over the ACQUITY UPLC BEH C18 columns. Ideally suited for analysis where a shorter retention time is required, the ACQUITY UPLC BEH C8 columns provide faster retention of nonpolar compounds, while maintaining chromatographic performance and improved peak shape delivery. The ACQUITY UPLC BEH C8 columns utilize trifunctional ligand bonding chemistries that guarantee low pH stability and negligible column bleed. Combined with the high pH stability of the sub-2 micron particles, (1.7 μm BEH particles, in this case), the specialized chemistries and low pH stability of the ACQUITY UPLC BEH C8 columns offer the most comprehensive pH operating range. Further, they employ exclusive end-capping processes and techniques to achieve exceptional peak shape for bases. The particle synthesis innovations and bonding chemistries result in the highest efficiencies, maximum MS sensitivities, and sharpest peak shapes. The improved chromatographic performance is highly desired for method developers in a variety of applications, as the hybrid particle substrate and reversed-phase separation mode boost this C8 column to a new standard of lower retention columns. The ACQUITY UPLC BEH C8 columns are designed to be used with the ACQUITY UPLC BEH C8 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk that attach directly to the inlet side of the UPLC column. The VanGuard pre-columns use the same frits and materials as the 2.1 mm ID UPLC columns, observing the same ISO 9001 standards to ensure peak performance. Shop for lab equipment and other related products with Waters’ online shop.

FAQ
What is the eCord Chip used for? The Waters eCord Intelligent Chip is an instrument that will record the column's performance throughout its lifetime. The chip will continue to maintain the column's history even when the column is removed from one instrument and attached to another. The chip will also carry the tracking and quality control information from the time of its manufacture, including mobile phases, running conditions, and analytes used to test the columns, removing the need for a paper Certificate of Analysis. Once the column is installed, key parameters are automatically downloaded into a column history file stored on the chip. What is BEH Technology? Playing a pivotal role in UPLC technology, the 1.7 µm BEH (Ethylene Bridged Hybrid) particle stands as a critical facilitator. It offers an array of pore sizes and bonded phases, enabling the application of reversed-phase and hydrophilic interaction chromatography. This cutting-edge technology finds its purpose across a wide spectrum, catering to the analysis of both small molecules and large molecule biopharmaceuticals.

Hybrid particle technology has intrinsic chemical stability, allowing for a wider usable pH range [pH 1-12]. This also enables a versatile, robust separation technology for method development.
The BEH particle technology is available in a variety of HPLC particle sizes [2.5, 3.5, 5, and 10 µm] which allows for a seamless transition between HPLC and UPLC technology platforms.