BRAND / VENDOR: Thermo Fisher

Thermo Fisher, 10361012, MAX Efficiency™ DH10Bac Competent Cells

CATALOG NUMBER: 10361012

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Product Description

MAX Efficiency™ DH10Bac Competent Cells MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The DH10BacE. colistrain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency DH10Bac Competent Cells include:
• Consistent yields of >1 × 108transformants/μg
• Tolerance of small amounts of undiluted ligation reactions
• Support of efficient blue/white colony screening (Φ80lacZΔM15 marker)Using MAX Efficiency DH10Bac competent cellsMAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and are capable of supporting site-specific recombination between pFastBac and bMON14272 to generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124.
Genotype:F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74recA1endA1araD139 Δ(ara,leu)7697galUgalK λ-rpsLnupG/pMON14272/pMON7124Bacterial or Yeast Strain: DH10Bac
Blue/White Screening: Yes
Cell Type: Chemically Competent
Cloning Methylated DNA: No
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Contains F' Episome: Lacks F' Episome
Efficiency: > 1x10^8
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Improves Plasmid Quality: No
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: DH10Bac, MAX Efficiency™
Product Type: Competent Cells
Propagating ccdB Vectors: Not for ccdB vector propagation
Quantity: 5 x 100 μL
Reduces Recombination: No
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: Medium Efficiency (10^8-10^9 cfu⁄μg)
Format: Tube
Species: E. coli
Unit Size: Each

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