Thermo Fisher, AM1926, RiboPure™ RNA Purification Kit, yeast

RiboPure™ RNA Purification Kit, yeast For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Ambion™ kit includes sufficient reagents for 50 purifications each from 107to 108yeast cells.
• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-free™ ReagentsIt has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure™-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Ambion's Vortex Bead Beater Adapter (see Accessory Products) followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.
05%). The kit has been used extensively at Ambion to isolate high-quality RNA fromSaccharomyces cerevisiae,Schizosaccharomyces pombe,Pichia pastoris, andCandida albicans(see Figure). The kit provides the highest yield of any method tested from each of these species (see Figure).
Accessory Products:The Kit requires the use of the Vortex Adapter (Cat. No. AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie™ 2 and holds 12 tubes.
Elution Volume: 50–100 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Purification Time: 1.
5 hr. (Including DNase treatment)
Quantity: 50 Preps
Shipping Condition: Box 1: Dry IceBox 2: Room Temperature
Starting Material Amount: Up to 108cells
Yield: Up to 300 μg
Isolation Technology: Spin Column (Glass Fiber Filter)
Sample Type: Yeast
Unit Size: Each