Thermo Fisher, F850S, Equine Genotypes Panel 1.1 Kit

100 Reactions Thermo Scientific Equine Genotypes Panel 1. 1 is a reagent kit for horse parentage testing and identification using short tandem repeats (STRs). The Equine Genotypes Panel 1. 1 provides all the necessary reagents for amplification of the 17 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Equine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Equine Genotypes Panel 1. 1. kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e. g. a conventionalTaqDNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'. One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument. The Equine Genotypes Panel 1. 1 allows co-amplification of the 17 microsatellites in a single multiplex PCR reaction. The Panel encompasses the following loci: VHL20, HTG4, AHT4, HMS7, HTG6, AHT5, HMS6, ASB23, ASB2, HTG10, HTG7, HMS3, HMS2, ASB17, LEX3, HMS1 and CA425. These include the nine loci recommended by the Equine Genetics and Thoroughbred Parentage Testing Standardization Committee of the International Society for Animal Genetics (ISAG) and eight additional loci commonly used for horse parentage testing and identification. Highlights
• Single multiplex PCR of 17 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)Detection Method: Primer-Probe
For Use With (Application): PCR
No. of Reactions: 100 Reactions
Product Type: Equine Genotypes Panel 1.1 Kit
Quantity: 100 reactions
Target: Equine Genotyping and Parentage Testing
Technique: STR (Short Tandem Repeat) Analysis
Unit Size: Each