BD, 550554, BD Pharmingen™ Biotin Mouse Anti-Mouse I-A[d]

Reactivity: Mouse (QC Testing)
Isotype: Mouse SJL IgG2b, κ
Immunogen: BALB/c mouse splenocytes
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 62.5 µg/ml
RRID: AB_393747
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The AMS-32.1 clone reactive against mouse I-A[d] is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse (BALB/c) spleen and thymus. The antibody stains the I-A[d] molecules found on the surface of cells expressing MHC-II in strains of mice expressing this haplotype. For optimal immunohistochemical staining, the biotinylated antibody should be titrated (1:10 to 1:50 dilution) and developed with Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone AMS-32.1 is not recommended for formalin-fixed paraffin embedded sections.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. An isotype control should be used at the same concentration as the antibody of interest. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.