Cytiva Affinity Antibody Resins for Robust mAb Purification

Purifying monoclonal antibodies efficiently is critical when you are moving projects from early development into reliable manufacturing. Cytiva’s affinity antibody resins, including MabSelect SuRe LX and MabSelect PrismA, are designed for high-capacity Protein A capture steps while supporting demanding cleaning protocols and process intensification.

Why Choose Cytiva Affinity Antibody Resins for mAb Capture

Selecting the right Protein A chromatography resin can determine how smoothly your downstream purification runs scale. You need confidence in binding capacity, alkaline stability, lifetime, and performance with high-titer feeds, without sacrificing product quality or regulatory readiness.

Cytiva’s affinity antibody resins are built for modern mAb processes, where upstream titers are often high and cleaning strategies rely on sodium hydroxide. They are designed to deliver high dynamic binding capacity, robust ligand stability, and consistent performance across development, scale-up, and commercial manufacturing. By using these resins in your capture step, you can simplify process development, reduce cost per gram of antibody, and create a more resilient downstream platform.

Product Portfolio at a Glance – MabSelect SuRe LX & MabSelect PrismA

Before you refine your process, it helps to see the available Protein A options side by side. The MabSelect SuRe family and MabSelect PrismA resin cover a range of performance needs, from high-capacity capture to enhanced alkaline stability for intensive cleaning and long resin lifetimes.

The table below summarizes the key Cytiva affinity antibody resins highlighted on this page, including catalog numbers, typical use cases, and core benefits. This overview can serve as a starting point when you evaluate which resin fits best with your upstream titers, cleaning strategy, and production scale.

Catalog number	Product name	Resin type	Typical scale	Key benefits
17547405	MabSelect SuRe LX, 10 L	Protein A affinity chromatography resin	Pilot and commercial manufacturing	High dynamic binding capacity, alkali-stable, suited for high-titer feeds
17547404	MabSelect SuRe LX, 5 L	Protein A affinity chromatography resin	Process development and small-scale manufacture	Same performance as 10 L packs; flexible for development and scale-up
17543804	MabSelect SuRe™ antibody purification resin	Protein A affinity chromatography resin	Lab and process development	Recombinant Protein A ligand, reliable platform for mAb capture
17549804	MabSelect PrismA, 5 L	Protein A chromatography resin	Process-intensified and commercial processes	Enhanced alkaline stability and lifetime, supports high CIP NaOH conditions

MabSelect SuRe LX – High-Capacity, Alkali-Stable Protein A Resin

When your upstream process delivers high-titer cultures, your capture step must keep pace without expanding column dimensions unnecessarily. MabSelect SuRe LX is designed for high binding capacity and robust alkaline stability, helping you maintain productivity while supporting rigorous column cleaning routines.

With its recombinant Protein A-derived ligand, MabSelect SuRe LX combines high dynamic binding capacity with good caustic stability. You can use sodium hydroxide-based cleaning in place, which supports microbial control and efficient sanitization. The resin is well-suited for large-scale antibody manufacturing, allowing you to load more mAb per cycle and potentially reduce the number of cycles needed per batch.

Key benefits you may leverage in your process include:

• High binding capacity for monoclonal antibodies from high-titer feeds
• Alkali-stable design enabling repeated NaOH cleaning-in-place
• Platform suitability across different antibody molecules and Fc-containing proteins
• Support for scale-up from development columns to production-scale columns

MabSelect SuRe Antibody Purification Resin – Platform Protein A for Development

Early development work often benefits from a robust yet versatile Protein A resin that supports many different antibody candidates. MabSelect SuRe antibody purification resin is built around a recombinant Protein A ligand that delivers reliable binding performance with good alkaline stability.

This resin supports efficient capture of IgG-class antibodies and other Fc-containing molecules, helping you establish platform methods that can be transferred to larger-scale operations. You can apply typical bind–wash–elute workflows and incorporate NaOH-based CIP strategies, which can simplify your cleaning validation.

Practical advantages you can use in your lab and pilot work include:

• Recombinant ligand for consistent batch-to-batch performance
• Compatibility with a range of buffer systems and pH conditions
• Support for repeated CIP with sodium hydroxide within recommended limits
• Suitable for screening, optimization, and small-scale production runs

MabSelect SuRe antibody purification resin is supplied under catalog number 17543804, giving you a defined, traceable resin identity when you document methods, material lists, and validation reports.

MabSelect PrismA – Next-Generation Protein A for Intensive Processes

As processes become more intense, with higher upstream titers and stricter cleaning requirements, you may need a Protein A resin that tolerates more aggressive conditions while preserving performance over many cycles. MabSelect PrismA is designed as a next-generation Protein A chromatography resin to meet these demands.

MabSelect PrismA incorporates an advanced Protein A-derived ligand and an optimized base matrix to improve alkaline stability and lifetime under NaOH cleaning regimes. This means you can implement robust CIP strategies while maintaining binding performance and minimizing ligand leakage. The resin is highly suitable for large-scale antibody production and process-intensified workflows where you aim to maximize resin utilization.

Typical advantages you may see when adopting MabSelect PrismA include:

• Enhanced alkaline stability to support higher NaOH concentrations and more cycles
• High binding capacity that supports high-titer feeds and efficient column loading
• Potential for longer resin lifetime, contributing to lower cost of goods
• Compatibility with platform mAb processes and regulatory expectations

How to Select the Right Affinity Antibody Resin

Choosing between MabSelect SuRe LX, MabSelect SuRe antibody purification resin, and MabSelect PrismA depends on how your process balances development flexibility, capacity needs, and cleaning stringency. A structured comparison helps you match resin properties with your upstream and downstream design.

When you evaluate your options, start with your upstream titer and expected feed volume, then consider your cleaning strategy and desired resin lifetime. High-titer processes may lean toward high-capacity resins to minimize column size. Processes that run many cycles or rely on strong NaOH cleaning for microbial control will benefit from resins engineered for enhanced alkaline stability.

The simple comparison below can guide your first selection:

Criterion	MabSelect SuRe antibody purification resin (17543804)	MabSelect SuRe LX (17547404 / 17547405)	MabSelect PrismA (17549804)
Primary focus	Development platform and small-scale purification	High-capacity capture for high-titer feeds	Next-generation alkaline stability and lifetime
Typical application	Screening and process development	Pilot and commercial manufacturing	Process-intensified and high-cycle commercial processes
Alkaline stability	Good	High	Enhanced
Dynamic binding capacity	High	Very high	Very high
Cleaning strategy	NaOH CIP within recommended conditions	Regular NaOH CIP with robust performance	More aggressive NaOH CIP and extended cycle numbers

You can refine this initial choice further by running small-scale scouting experiments, comparing performance across your specific feeds and target quality attributes.

Typical Applications and Downstream Workflow Integration

Affinity antibody resins sit at the heart of most mAb downstream workflows. Understanding how they integrate with other steps helps you design a purification train that delivers the purity, yield, and throughput you need.

In a typical monoclonal antibody process, the Protein A capture step comes immediately after harvest and clarification. The chosen Cytiva affinity antibody resin binds the Fc region of your antibody, concentrating and purifying it in a single step. The eluate then usually proceeds to polishing steps such as ion exchange chromatography, hydrophobic interaction chromatography, and virus clearance or nanofiltration. Finally, ultrafiltration/diafiltration (UF/DF) is used for concentration and buffer exchange into the final formulation.

A simplified workflow might look like this:

1. Cell culture harvest and clarification
2. Protein A capture on MabSelect SuRe LX, MabSelect SuRe resin, or MabSelect PrismA
3. Polishing steps (e.g., ion exchange and hydrophobic interaction chromatography)
4. Virus filtration and sterile filtration
5. UF/DF concentration and formulation

By pairing Cytiva affinity antibody resins with compatible ion exchange resins, filtration devices, and buffer systems, you can build a coherent platform that is easier to scale and validate.

FAQs

1. How do cleaning conditions affect resin lifetime?

Resin lifetime is highly influenced by the concentration, exposure time, and frequency of NaOH cleaning cycles. Using resins designed for alkaline stability, such as MabSelect SuRe LX and MabSelect PrismA, allows you to apply effective CIP strategies while maintaining performance over many cycles within the recommended operating guidelines.

2. Can these resins handle different antibody subclasses or Fc-fusion proteins?

Cytiva’s affinity antibody resins are designed to bind IgG-class antibodies and many Fc-containing molecules. However, binding strength can vary between subclasses and constructs. You may want to run small-scale studies to confirm binding capacity and elution conditions for each new molecule.

3. How should I approach scale-up from development to manufacturing columns?

Start by defining your target load per cycle, residence time, and feed properties at small scale. Once you have a robust method on development columns packed with the same resin family, you can scale up column dimensions while maintaining comparable linear velocities and residence times, validating performance at each step.