Cytiva MabSelect Protein A Resins for Antibody Purification

Choosing the right Protein A resin is one of the fastest ways to improve your monoclonal antibody (mAb) purification workflow. Cytiva’s MabSelect™ family combines high dynamic binding capacity, robust alkaline stability, and scalability from process development to commercial manufacturing, giving you a reliable platform for capture steps.

Why Protein A Resin Choice Matters in mAb Purification

As upstream titers continue to rise, your Protein A capture step carries more load than ever before. The resin you select directly affects yield, impurity removal, clean-in-place (CIP) strategy, and lifetime cycles, which in turn drive cost of goods, throughput, and batch-to-batch consistency in your downstream process.

When you evaluate Protein A media, you are really balancing three factors: dynamic binding capacity, alkaline stability, and process economy. MabSelect SuRe™, MabSelect SuRe™ LX, and MabSelect™ PrismA are designed to address these needs at different scales and performance levels, so you can match resin performance to your process stage instead of accepting one-size-fits-all compromises.

Cytiva MabSelect Protein A Resin Series Overview

Across the MabSelect family, you get a rigid agarose base matrix, oriented Protein A–A-derived ligands, and high flow properties that support both packed-bed columns and modern high-titer feeds. All three resins withstand repeated CIP with NaOH, offering strong alkaline stability and long usable lifetimes for industrial workflows.

At a high level, you can think of the series as an evolution of performance:

• MabSelect SuRe – Alkali-stable Protein A resin for reliable, cost-effective mAb capture.
• MabSelect SuRe LX – Very high DBC for high-titer feeds and process-scale capacity gains.
• MabSelect PrismA – Next-generation Protein A resin with enhanced binding capacity and alkaline stability for future-ready mAb processing.

MabSelect SuRe™ Antibody Purification Resin

MabSelect SuRe is often the starting point for robust Protein A capture: it combines an alkali-tolerant, engineered Protein A ligand with a rigid agarose matrix to deliver stable performance across many CIP cycles. If you need dependable performance and controlled ligand leakage, SuRe provides a proven base for clinical and commercial processes.

Key Features

• Recombinant, alkali-tolerant Protein A-derived ligand designed to withstand 0.1–0.5 M NaOH CIP.
  
• High dynamic binding capacity suitable for typical mAb titers and residence times.
  
• Rigid high-flow agarose matrix supporting good pressure–flow properties.
  
• Reduced ligand leakage compared with earlier-generation Protein A resins, helping protect product quality.
  

Typical Applications

• Research and process development runs where robustness and ease of method transfer are key.
• Clinical manufacturing lines that need consistent performance over many cycles.
• Capture steps where you want a strong process economy but do not yet face extreme upstream titers.

MabSelect SuRe LX – High-Capacity Resin for Large-Scale mAb Capture

As titers and production scales increase, you may need a resin that provides higher mass throughput per column volume without sacrificing alkaline stability. MabSelect SuRe LX builds on the SuRe platform, adding significantly increased dynamic binding capacity at extended residence times to help you reduce column size or increase productivity.

Why Choose MabSelect SuRe LX

• Very high DBC at long residence times, ideal for high-titer mAb feeds.
  
• Alkali-tolerant Protein A ligand supports routine CIP with 0.1–0.5 M NaOH, preserving resin lifetime.
  
• Rigid agarose matrix with excellent pressure–flow properties for process-scale columns.
  
• Enables either higher throughput on existing hardware or downsized columns for the same load.
  

Recommended Use Cases

• Large-scale commercial processes that must handle high-titer cultures efficiently.
• Facilities seeking to reduce resin volume and related costs while maintaining output.
• Lines where improving productivity (g/L resin per year) is a central performance metric.

MabSelect PrismA – Next-Generation Protein A Resin

If your process must accommodate very high titers, advanced mAb formats, or stringent regulatory expectations, MabSelect PrismA gives you the highest performance within the MabSelect family. It combines an optimized high-flow agarose base with a newly engineered Protein A–derived ligand, delivering significantly enhanced binding capacity and alkaline stability for long-term, intensive use.

Enhanced Performance with PrismA

• Next-generation Protein A ligand designed for very high DBC, supporting high mass throughput per resin volume.
  
• Significantly improved alkaline stability to withstand repeated NaOH CIP cycles and extended lifetimes. 
• Optimized base matrix engineered for high flow and low backpressure in production-scale columns.
  
• Suitable for a range of antibody formats, including challenging or bispecific mAbs, depending on binding behavior.
  

Ideal Scenarios

• Commercial mAb facilities require maximum process robustness and throughput.
• Processes that are being intensified (higher titers, fewer cycles) need extra binding capacity.
• Workflows where regulatory and quality expectations leave little margin for resin degradation.

Side-by-Side Comparison: MabSelect SuRe vs SuRe LX vs PrismA

When you compare the three resins side by side, the progression from robust baseline performance to high-capacity and next-generation capability becomes clear. Instead of choosing on name alone, you can align resin choice with your specific titer, scale, and lifecycle requirements.

Performance Snapshot

Parameter	MabSelect SuRe	MabSelect SuRe LX	MabSelect PrismA
Binding capacity (qualitative)	High	Very high	Highest within the MabSelect family
Alkaline stability (NaOH CIP)	0.1–0.5 M NaOH; robust CIP	0.1–0.5 M NaOH; process-scale lifetimes	Significantly enhanced alkaline stability
Matrix	High-flow agarose	High-flow agarose	Optimized high-flow agarose
Typical use	R&D, clinical, smaller-scale mAb	High-titer feeds, large-scale commercial	Future-ready, intensified commercial processes

Representative SKUs

To help you align resin choice with ordering, below are examples of popular Cytiva catalog numbers frequently used for bulk resin packaging:

Resin	Catalog Number	Pack Size	Description
MabSelect SuRe	17543802	200 mL	MabSelect SuRe antibody purification resin
MabSelect SuRe LX	17547403	1 L	MabSelect SuRe LX Protein A resin
MabSelect PrismA	17549805	10 L	MabSelect PrismA Protein A chromatography resin

These catalog numbers match Cytiva product listings and are available through multiple distributors, including their appearance on iRight and major lab supply channels.

Application Scenarios and Selection Guide

Different teams will prioritize different performance levers. A development lab optimizing a new molecule does not face the same constraints as a commercial facility running multiple high-titer campaigns. This simple selection guide helps you map your process context to the most suitable MabSelect resin.

Scenario / Priority	MabSelect SuRe	MabSelect SuRe LX	MabSelect PrismA
Early R&D and method development	Best fit	Also suitable	Typically not required
Clinical-scale manufacturing	Strong option	Strong option	For demanding processes
High-titer commercial production	May be capacity-limited	Best fit	Best fit for future expansion
Maximum throughput and minimal resin volume	Strong option	Best fit	Best fit
Most stringent CIP / lifetime expectations	Good	Very good	Highest

In practice, you might begin with MabSelect SuRe in early development, shift to MabSelect SuRe LX as titers and scale increase, and adopt MabSelect PrismA when you intensify processes further or when you need the largest possible performance margin under regulatory pressure.