BD, 553070, BD Pharmingen™ Biotin Rat Anti-Mouse CD25

Alternative Name: IL-2 Receptor α chain, p55
Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgM, κ
Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394602
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures For detection of low-density CD25 expression, we recommend the use of a "bright" second-step reagent, such as PE Streptavidin (Cat. No. 554061). For immunohistochemical staining, we recommend the use of Biotin Rat Anti-Mouse CD25 (Cat. No. 550529) in our special formulation for immunohistochemistry.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Use of these products to measure activation antigens expressed on mononuclear cell subsets for the purpose of monitoring immunoregulatory status can fall under one or more claims of the following patents: US Patent Nos. 5,445,939, 5,656,446, 5,843,689; European Patent No. 319,543; Canadian Patent No. 1,296,622; Australian Patent No. 615,880; and Japanese Patent No. 2,769,156. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.