Agilent, 5190-2425, Agilent Bio SCX, NP5, 4.6 x 250 mm

Agilent Bio SCX, NP5, 4.6 x 250 mm. A strong cation exchange HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio SCX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: SCX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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This high-performance strong cation exchange (SCX) column features a 4.6 x 250 mm format and employs non-porous 5-micron particles for optimal protein and peptide separation. Engineered for robust chromatographic workflows, it enables efficient fractionation and analysis of complex biological samples, making it ideal for proteomics research, biomolecule characterization, and the identification of post-translational modifications. The column delivers excellent resolution, sharp peak shapes, and consistent performance, even when processing samples with high ionic strength. Designed for compatibility with HPLC and UHPLC platforms, it integrates seamlessly into Agilent’s 1200 Infinity and 1260 Infinity II systems, as well as a wide range of LC instruments from other manufacturers. Suitable for analytical and preparative applications, this SCX solution supports scalable purification, in-depth protein profiling, and the isolation of specific target molecules needed in pharmaceutical development and quality control laboratories. Optimized for reproducibility, it works effectively alongside complementary Agilent reversed-phase, size exclusion, and affinity columns for multidimensional LC workflows. Researchers benefit from enhanced productivity and reliable results when employing this solution-based approach for routine monitoring, complex separations, or advanced bioanalytical studies.