Agilent, 5190-2443, Agilent Bio WCX, NP3, 4.6 x 50 mm

Agilent Bio WCX, NP3, 4.6 x 50 mm. A weak cation exchange HPLC column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WCX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 50 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 3 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 551 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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This high-performance weak cation exchange (WCX) chromatography column features non-porous, 3-micron particles, and dimensions of 4.6 x 50 mm, providing excellent resolution and fast separations for biomolecule analysis. Its advanced resin technology supports highly selective and reproducible separation of proteins, peptides, and other positively charged biomolecules based on ionic interactions, making it especially suitable for the characterization of monoclonal antibodies, charge variant analysis, and protein isoform profiling.

The column is designed for robust operation with low nonspecific binding, ensuring reliable results for both analytical and preparative work. Compatible with a broad range of aqueous mobile phases and salt gradients, it integrates seamlessly with Agilent 1100, 1200, and Infinity series HPLC and UHPLC systems, and is also suitable for use with comparable LC platforms from other manufacturers. Its durable design supports high-throughput laboratory environments, including biopharmaceutical development, quality control, and academic research.

Additionally, it pairs well with Agilent's wide range of bio-inert LC hardware, sample preparation products, and associated protein analysis consumables, providing a comprehensive solution for charge-based biomolecule separations. This column is an excellent choice for laboratories seeking reproducible, high-resolution results in demanding protein and peptide analysis workflows.