Agilent, 5190-2464, Agilent Bio SAX, NP3, 4 x 10 mm, guard

Agilent Bio SAX, NP3, 4 x 10 mm, guard. A strong anion exchange HPLC guard column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio SAX
Column Material: Stainless Steel
Guard Column: Yes
Hardware: SS
Inner Diameter (ID): 4 mm
LC Platform: Stainless Steel
Length: 10 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 3 µm
Particle Type: Non-porous
Phase: SAX
Pressure Rating: 551 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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This guard column is designed to provide robust protection for analytical columns used in biologic and protein separations via strong anion exchange chromatography. Engineered with NP3 packing material and sized at 4 x 10 mm, it effectively safeguards the main chromatographic column from sample particulates and contaminants, extending operational lifetime and maintaining high analytical performance. Its composition and dimensions ensure minimal dead volume and preserve chromatographic resolution when paired with comparable columns in biochromatography workflows.

Ideal for laboratories conducting detailed characterization and quantification of monoclonal antibodies, vaccines, therapeutic proteins, and other complex biomolecules, this guard column offers reliable retention and reproducibility. It integrates seamlessly with Agilent’s Bio SAX columns and is compatible with a wide range of high-performance liquid chromatography HPLC and UHPLC systems, including Agilent LC instrument platforms.

The product is particularly suitable for process development, quality control, and analytical research environments where sample cleanliness and column integrity are critical for consistent results. For optimal performance, it is recommended to use this guard column alongside matched separation columns in regulated biopharmaceutical, academic, or industrial research settings. Related consumables such as Bio SAX main columns and connection kits are available to support comprehensive system compatibility and workflow efficiency.