Agilent, 5190-2483, Agilent Bio WAX, NP3, 4.6 x 50 mm.

Agilent Bio WAX, NP3, 4.6 x 50 mm. A weak anion exchange HPLC column with nonporous particles. Particle size is 3 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WAX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 50 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 3 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 551 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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Designed for high-resolution separation of biomolecules, this Bio WAX column with NP3 chemistry offers a strong anion-exchange stationary phase ideal for analytical and research laboratories. With dimensions of 4.6 x 50 mm, it enables efficient analysis of proteins, peptides, oligonucleotides, and other acidic biological compounds. The column’s advanced non-porous particle technology ensures rapid mass transfer, delivering sharp peak shapes and reproducible retention times, even with complex biological samples.

Optimized for high-throughput workflows, it integrates seamlessly with Agilent 1100, 1200, and Infinity Series HPLC systems, as well as most standard LC instrumentation. Researchers can confidently employ this column in applications such as protein characterization, quality control of biotherapeutics, and charge variant analysis in the pharmaceutical and biotechnology sectors. Its robust construction supports consistent performance across varied buffer conditions and repetitive uses, minimizing downtime and maintenance.

For expanded application coverage, it is fully compatible with Agilent’s Bio HPLC column portfolio and a range of validated consumables and solvents, simplifying method development and validation. This makes it a reliable choice for laboratories seeking reproducibility and high sensitivity in bioseparation workflows.