Agilent, 5190-2486, Agilent Bio WAX, NP5, 4 x 10 mm, guard

Agilent Bio WAX, NP5, 4 x 10 mm, guard. A weak anion exchange HPLC guard column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WAX
Column Material: Stainless Steel
Guard Column: Yes
Hardware: SS
Inner Diameter (ID): 4 mm
LC Platform: Stainless Steel
Length: 10 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WAX, NP5, 4 x 10 mm, guard is a high-performance guard column designed for optimal protection and extension of analytical column lifetime in ion-exchange chromatography applications. Featuring a non-porous, weak anion exchange (WAX) stationary phase, this guard is ideal for safeguarding the main column from contaminants and particulates that can compromise chromatographic performance during complex biomolecule analyses. Its robust NP5 particle technology offers excellent resistance to fouling, ensuring reliable separation and consistent results over extended use.

Typical applications include the analysis of monoclonal antibodies, oligonucleotides, proteins, and other charged biomolecules in pharmaceutical, biotechnology, and academic research environments. The guard’s dimensions make it suitable for integration into standard bio-inert HPLC and UHPLC systems, supporting smooth workflows and decreasing downtime associated with column maintenance. It is fully compatible with Agilent Bio WAX analytical columns, providing seamless operation and method reproducibility.

By utilizing this guard column, researchers can confidently perform sensitive ion-exchange separations with improved sample integrity and enhanced instrument protection. Its tool-free, leak-free cartridge design streamlines installation and replacement, making it an essential component for robust and reliable bioseparation workflows.