Agilent, 5190-2488, Agilent Bio WAX, NP5, 4.6 x 50 mm, PEEK

Agilent Bio WAX, NP5, 4.6 x 50 mm, PEEK. A weak anion exchange HPLC column with nonporous particles. Particle size is 5 μm. PEEK hardware enables this column to couple with bio-compatible or bio-inert LC systems.

Specifications:
Brand: Agilent Bio WAX
Column Material: PEEK
Guard Column: No
Hardware: PEEK
Inner Diameter (ID): 4.6 mm
LC Platform: BioInert
Length: 50 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WAX NP5 4.6 x 50 mm PEEK column is specifically engineered for high-resolution anion exchange chromatography of biomolecules. Featuring a non-porous 5-micron particle design and robust PEEK hardware, this column enables rapid separation and analysis of proteins, peptides, and nucleic acids, delivering reliable performance with minimized risk of sample adsorption or metal interaction. Its high efficiency makes it ideal for analytical and semi-preparative workflows in biopharmaceutical development, quality control, and academic research laboratories.

Optimized for compatibility with aqueous mobile phases, the column supports direct analysis of complex biological samples such as therapeutic proteins, monoclonal antibodies, and oligonucleotides. It is well-suited for applications involving charge variant analysis, glycoprotein profiling, and process monitoring of biotherapeutics. The chemically inert PEEK construction allows for safe use with biological samples and aggressive buffers, preserving sample integrity and column longevity.

This column integrates seamlessly with Agilent 1200 Infinity and 1260 Infinity II LC systems, as well as other standard HPLC platforms. It pairs effectively with Agilent’s range of bio-inert LC accessories and detectors, ensuring accurate, reproducible results across bioresearch and biomanufacturing environments.