Agilent, 5190-2493, Agilent Bio WAX, NP10, 4.6 x 250 mm

Agilent Bio WAX, NP10, 4.6 x 250 mm. A weak anion exchange HPLC column with nonporous particles. Particle size is 10 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WAX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 4.6 mm
LC Platform: Stainless Steel
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: OligosProteins
Particle Size: 10 µm
Particle Type: Non-porous
Phase: WAX
Pressure Rating: 275 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-12

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The Agilent Bio WAX NP10, 4.6 x 250 mm column is designed for superior ion exchange chromatography, offering robust performance for the separation of proteins, peptides, and other biomolecules. Featuring a non-porous polymeric substrate, this weak anion exchange column delivers exceptional resolution, peak symmetry, and fast mass transfer, making it ideal for high-throughput analytical and preparative workflows in biopharmaceutical research and quality control.

With dimensions of 4.6 mm inner diameter and 250 mm length, it enables precise separation and quantitation of charged analytes from complex biological samples, supporting the characterization, purity assessment, and charge variant analysis of monoclonal antibodies, recombinant proteins, and other biologics. This column is fully compatible with Agilent HPLC and UHPLC systems, including the 1260 and 1290 Infinity series, as well as other leading liquid chromatography platforms. Its robust chemistry ensures chemical stability and consistent reproducibility across multiple batch runs.

Optimal for use in laboratories engaged in therapeutic protein characterization, vaccine development, and bioprocess monitoring, it is a reliable solution for researchers requiring high sensitivity and reproducibility in biopharma, proteomics, and life sciences applications. For expanded analytical flexibility, it can be paired with complementary Agilent Bio SEC or reversed-phase columns.