Agilent, 5190-6879, Agilent Bio SCX, NP5, 21.2 x 250 mm, 5 µm

Agilent Bio SCX, NP5, 21.2 x 250 mm, 5 µm. A strong cation exchange prep sized HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio SCX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: SCX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-12

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The Agilent Bio SCX NP5 column, dimensioned at 21.2 x 250 mm with 5 micron particle size, is engineered for high-resolution separation of proteins and biomolecules through strong cation exchange chromatography. Its robust silica matrix and uniform particle size deliver exceptional efficiency, reproducibility, and sample recovery, making it suitable for preparative and semi-preparative scale purification. Designed for optimal separation of complex protein mixtures, charge variants, and antibody drug conjugates, this column is widely utilized in biopharmaceutical research, quality control, and process development.

It is compatible with most standard high-performance liquid chromatography HPLC and preparative chromatography systems, ensuring flexibility across various laboratory platforms. This column provides excellent chemical stability and consistent performance under a wide range of mobile phase conditions, making it suitable for both analytical method development and scale-up applications. For efficient purification workflows, it can be integrated with Agilent’s portfolio of HPLC systems and complemented by other biochromatography products such as reversed-phase and size exclusion columns. Researchers, analytical scientists, and process developers benefit from its ability to resolve challenging biomolecules, ensuring accurate characterization and high-purity isolation vital for downstream applications including therapeutic protein development and detailed structural studies.