Agilent, 5190-6880, Agilent Bio WCX, NP5, 10 x 250 mm, 5 µm

Agilent Bio WCX, NP5, 10 x 250 mm, 5 µm. A weak cation exchange semi-prep sized HPLC column with nonporous particles. Particle size is 5 μm. Nonporous particles are best for efficient mass transfer and high resolution separations.

Specifications:
Brand: Agilent Bio WCX
Column Material: Stainless Steel
Hardware: SS
Inner Diameter (ID): 10 mm
LC Platform: Preparative HPLC
Length: 250 mm
Maximum Temperature: 80 °C
Part Number Application Domain: Biopharma
Part Number Application: Charge Variant AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Non-porous
Phase: WCX
Pressure Rating: 413 bar
Separation Mode: Ion Exchange (IEX)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-12

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The Agilent Bio WCX, NP5, 10 x 250 mm, 5 µm column is designed for robust and high-resolution separation of proteins and peptides based on their weak cation exchange properties. Engineered with 5-micron particle size and a 10 x 250 mm format, this column delivers consistent performance and reliable reproducibility, making it ideal for complex biomolecule analysis in research and biopharmaceutical quality control. Its narrow pore structure supports efficient separation of large biomolecules, including monoclonal antibodies, glycoproteins, and enzyme variants. Typical usage scenarios include protein purification, charge variant analysis, and characterization of post-translational modifications. This column is fully compatible with Agilent 1100, 1200, and 1260 Infinity LC systems, as well as other major HPLC platforms that accept standard 10 mm diameter columns. It operates with commonly used buffer systems for cation exchange chromatography, providing flexibility in method development and scalability from analytical to semi-preparative workflows. The Agilent Bio WCX column ensures high sample recovery, low carryover, and excellent peak shape, supporting rigorous requirements in proteomics, academic research, pharmaceutical, and biotechnology laboratories. Related products include other Agilent Bio IEX columns, sample preparation kits, and compatible guard columns for extended column lifetime.