Agilent, GKE-5016B, AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL

AdvanceBio N-Glycanase (PNGase F, EDTA free), ≥2.5 U/mL. A recombinant form of PNGase F (200 mU, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution, 5x N-glycanase Tris reaction buffer (MS applications).

Specifications:
Concentration: 2.5 U/mL
Enzyme Formulation: 20 mM Tris-HCl, 50 mM NaCl (pH 7.5)
Enzyme Source: Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase.
Enzyme Specific Activity: ≥10 units/mg
Enzyme Specificity: Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition: One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 µmole of denatured ribonuclease B per minute at 37°C, pH 7.5.
Molecular Weight: ~35,000 Daltons
Unit: 200 mU
Volume: 80 µL
pH Optimum: 8.6
pH Range: 7.5-9.5

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AdvanceBio N-Glycanase is a high-purity recombinant enzyme formulated for efficient and complete release of N-linked glycans from glycoproteins. This EDTA-free preparation has an activity of at least 2.5 units per milliliter, ensuring reliable performance in sensitive biopharmaceutical and glycoprotein characterization workflows. Its compatibility with both native and denatured proteins makes it suitable for a broad range of research and development applications, including therapeutic monoclonal antibody analysis, vaccine glycosylation profiling, and glycan structure elucidation.

This enzyme streamlines sample preparation for downstream techniques such as liquid chromatography, mass spectrometry, and capillary electrophoresis. It is ideally suited for high-throughput laboratories working with Agilent AdvanceBio Glycan Mapping columns, as well as other popular systems for glycan analysis. The absence of EDTA broadens its compatibility with metal-sensitive assays and proteomics workflows, minimizing enzyme interference and preserving native protein modifications.

AdvanceBio N-Glycanase is recommended for researchers needing robust, reproducible deglycosylation in compliance with rigorous analytical standards. Used as part of glycan profiling kits or with automated sample preparation systems, it enables confident and reproducible N-glycan release for qualitative and quantitative studies across pharmaceutical, biotechnology, and academic settings.