BD, 340031, BD Simultest™ Anti-Human CD8 FITC/CD28 PE

Reactivity: Human
Application: Flow cytometry
Regulatory Status: RUO (GMP)
RRID: AB_399998
Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.
Description: Description CD8, clone SK1, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. CD28, clone L293, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with spleen cells from BALB/c mice immunized with the HPB-ALL T-cell line. The CD8 (Leu-2a) antigen is present on the human suppressor/cytotoxic T-lymphocyte subset as well as on a subset of natural killer (NK) lymphocytes. The CD8 antigenic determinant interacts with class I major histocompatibility complex (MHC) molecules resulting in increased adhesion between the CD8 + T lymphocytes and the target cells. Binding of the CD8 antigen to class I MHC molecules enhances the activation of resting T lymphocytes. CD8 recognizes an antigen expressed on the 32-kdalton (kDa) α-subunit of a disulfide-linked bimolecular complex. The cytoplasmic domain of the α-subunit of the CD8 antigen is associated with the protein tyrosine kinase p56 lck . The CD28 antigen, a disulfide-linked homodimeric glycoprotein (Mr 44 kDa), is a cell adhesion molecule (CAM) and functions as the ligand for the B7/BB-1 antigen that is present on activated B lymphocytes. Interaction of the CD28 antigen with the B7/BB-1 antigen costimulates CD2 and CD3 antigen/T-cell antigen receptor (TCR)–dependent T-cell–mediated cytotoxicity.
Preparation And Storage: Preparation And Storage The Simultest reagent is supplied as a combination of CD8 FITC and CD28 PE in 1.0 mL of phosphate-buffered saline. Buffered saline contains gelatin and 0.1% sodium azide. The vial should be stored at 2° to 8°C and protected from prolonged exposure to light. Simultest reagents should not be frozen. Each Simultest reagent is stable for the period shown on the bottle label when stored as directed.
Description : Clone : Isotype : EntrezGene ID
N/A : N/A : | IgG1, κ,IgG1, κ : N/A