BD, 550368, BD Pharmingen™ Purified Mouse Anti-Human CD3

Alternative Name: CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 62.5 µg/ml
Workshop Number: I WT3; III 471
RRID: AB_393639
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures For optimal indirect immunohistochemical staining, the UCHT1 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880).