BD, 550527, BD Pharmingen™ BD Retrievagen B (pH 9.5)

Preparation And Storage: Preparation And Storage Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures BD Retrievagen B (pH 9.5) is provided as a ready to use solution. Antigen retrieval procedure is performed after deparaffinizing the slides as outlined below. 1.   Deparaffinize slides (after drying thoroughly overnight at room temperature) in 2 changes of xylene for 10 min each. 2.   Wash slides in 100% alcohol twice for 2 min each. 3.   Block endogenous peroxidase activity by incubating for 10 min in 3% H2O2. Rinse 2-3 times in water. 4.   Place slides in a plastic coplin jar or staining dish with the BD Retrievagen B solution and heat to 193° F in a microwave. 5.   Mix the solution and incubate the slides at the desired temperature of 193° F for 5 min. 6.   Remove the staining dish, cover and allow the slides to slowly cool to room temperature for 10 min. 7.   Wash slides with 2-3 changes of water and continue with the blocking with normal serum and application of the primary antibody.