BD, 550529, BD Pharmingen™ Biotin Rat Anti-Mouse CD25

Alternative Name: IL-2 Receptor α chain, p55
Reactivity: Mouse (QC Testing)
Isotype: Rat LEW, also known as Lewis IgM, κ
Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 125 µg/ml
RRID: AB_2125455
Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, goat serum, and ≤ 0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The 7D4 antibody specific for mouse CD25 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen and thymus. The antibody stains activated T and B lymphocytes and immature thymocytes. The isotype control recommended for use with this antibody is Biotin Rat IgM, κ Isotype Control (Cat. No. 550343). For optimal indirect immunohistochemical staining, the biotin format of the 7D4 antibody should be titrated (1:10 to 1:50 dilution) and visualized by Streptavidin HRP (Cat. No. 550946) together with the DAB Substrate Kit (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure can  be found on our website at http://www.bdbiosciences.com/us/s/resources. The clone 7D4 is not recommended for form alin-fixed paraffin embedded sections.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Use of these products to measure activation antigens expressed on mononuclear cell subsets for the purpose of monitoring immunoregulatory status can fall under one or more claims of the following patents: US Patent Nos. 5,445,939, 5,656,446, 5,843,689; European Patent No. 319,543; Canadian Patent No. 1,296,622; Australian Patent No. 615,880; and Japanese Patent No. 2,769,156. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).