BD, 550550, BD Pharmingen™ Biotin Mouse Anti-Mouse H-2K[b]

Reactivity: Mouse (QC Testing)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Mouse C57BL/6 Splenocytes
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-paraffin (Not Recommended)
Concentration: 125 µg/ml
RRID: AB_393744
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Recommended Assay Procedures: Recommended Assay Procedures The AF6-88.5 clone reactive against mouse H-2Kb is recommended to test for immunohistorical staining of acetone-fixed frozen sections. Tissues tested were mouse (C57B1/6) spleen and thymus. The antibody stains the H-2Kb molecules found on the surface of all nucleated cells in strains of mice expressing this haplotype. For optimal immunohistochemical staining, the biotinylated antibody should be titrated (1:10 to 1:50 dilution) and developed with Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone AF6-88.5 is not recommended for formalin-fixed paraffin embedded sections.