BD, 550556, BD Pharmingen™ Purified Rat Anti-Mouse MAdCAM-1

Reactivity: Mouse (QC Testing)
Isotype: Rat WI, also known as Wistar (outbred) IgG2a, κ
Immunogen: Mouse endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes.
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 31.25 µg/ml
RRID: AB_393749
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The MECA-367 antibody specific for mouse MadCAM-1 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissues tested were mouse spleen, thymus and small intestine. The antibody stains mucosal lymphoid tissue and lamina propria. In the spleen it is expressed in the sinus lining cells. The isotype control recommended for use with this antibody is purified rat IgG2a (Cat. No. 559073). For optimal indirect immunohistochemical staining, the MECA-367 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-rat Igs (multiple adsorbed) (Cat. No. 559286) as the secondary antibody and Streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). A detailed protocol of the immunohistochemical procedure is available on our website: www.bdbiosciences.com/support/resources .