BD, 550733, BD Pharmingen™ Purified Rabbit Anti-Human CD88

Alternative Name: C5a Receptor
Reactivity: Human (QC Testing)
Isotype: Rabbit IgG
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Tested During Development)
RRID: AB_393854
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: The purified C85-2506 clone can be used for immunohistochemistry to detect expression of the human C5aR. The antibody has been tested on frozen and fixation of paraffin sections of human tonsil and paraffin sections of human spleen, liver, colon and lung. The dilution range recommended for this antibody is 1-10 to 1-20. The C85-2506 clone does not cross react with C5aR expressed on mouse, rat, pig or monkey tissue. Using biotinylated donkey anti-rabbit Ig (Jackson ImmunoResearch) as the secondary antibody, followed by a peroxidase-based detection system, the expression of C5aR was observed on dendritic cells, granulocytes and monocytes of human tonsil.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.