BD, 551462, BD Pharmingen™ Purified Mouse Anti-Human CD126

Alternative Name: Interleukin 6 Receptor alpha chain; IL-6R alpha; IL-6Rα
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: CD126 Recombinant Protein
Application: Flow cytometry (Routinely Tested), ELISA Capture (Tested During Development)
Concentration: 0.5 mg/ml
Workshop Number: VI C63; IX 36
RRID: AB_394209
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The polyclonal antibody was purified from antiserum by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The Purified Mouse Anti-Human CD126 (Cat. No. 551462) can be used for the immunofluorescent staining and flow cytometric analysis of human nucleated cells to measure their expressed levels of surface IL-6Rα. An appropriate purified immunoglobulin isotype control is Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746). A three-layer staining protocol is recommended for maximizing the detection of IL-6Rα expressed by cells as detailed in the figure legend. ELISA: The Purified Mouse Anti-Human CD126 (Cat. No. 551462) can be used as the capture antibody, with recombinant soluble IL-6Ra as the standard, the Biotin Mouse Anti-Human CD126 (Cat. No. 552503) as a detection antibody for a sandwich ELISA measuring human IL-6Ra protein levels. Purified Mouse Anti-Human CD126 antibody should be titrated between 0.5-2 μg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human soluble IL-6Ra, ranging from 500 to 2 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our website, http://www.bdbiosciences.com/us/s/resources, and go to the protocols section under "ELISA and ELISPOT".