BD, 553445, BD Pharmingen™ Purified Rat Anti-Mouse IgG1

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Pooled mouse IgG1
Application: ELISA (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_394863
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures ELISA: The Purified Rat Anti-Mouse IgG1 antibody (clone A85-3) may be used as a capture reagent with Biotin Rat Anti-Mouse IgG1 (clone A85-1) (Cat. No. 553441) and Avidin-HRP (Cat. No. 554058) or Streptavidin-HRP (Cat. No. 554066) as detection reagents.  Alternatively,  investigators may also wish to consider using AKP Rat Anti-Mouse IgG1 (Cat. No. 557272) or HRP Rat Anti-Mouse IgG1 (Cat. No. 559626) for detection.  Purified Mouse IgG1 (Cat. No. 557273) should be used as the protein standard.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.