BD, 553712, BD Pharmingen™ Purified Rat Anti-Mouse CD138

Alternative Name: SYND1; Syndecan-1; syn-1; Sdc1; Sstn; synstatin
Reactivity: Mouse (QC Testing)
Isotype: Rat F344, also known as Fischer, CDF IgG2a, κ
Immunogen: NAMRU mouse mammary gland epithelial cell line NMuMG
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), ELISA, Immunohistochemistry-paraffin, Western blot (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394998
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures CD138 is expressed at low density on cells in the normal bone marrow; therefore, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a "bright" third-step reagent, such as Streptavidin-PE (Cat. no. 554061). Other reported applications include western blot analysis using a cationic blotting membrane and a specific method,  ELISA, and immunohistochemical staining of acetone-fixed frozen and paraffin-embedded sections.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.