BD, 554147, BD Pharmingen™ Purified Mouse Anti-p53

Reactivity: Monkey (QC Testing), Human, Mouse, Rat, Hamster (Reported)
Isotype: Mouse IgG2b
Immunogen: SV40-transformed mouse cell line BALB/c 3T3 clone 4 (B4)
Application: Western blot (Routinely Tested), Flow cytometry, Immunofluorescence, Immunoprecipitation (Tested During Development)
Target Molecular Weight: 53 kDa
Concentration: 0.5 mg/ml
RRID: AB_395267
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include immunoprecipitation (1-2 µg/1x10e6 cells), western blot analysis (2 µg/ml), immunofluorescence microscopy of cultured cells and flow cytometry. COS-7 SV40 transformed monkey kidney cells (ATCC CRL-1651) or another SV40-transformed cell line are suggested as positive controls for detecting p53. COS-7 cell lysate is optimized for a western blot control and comes ready to load on an SDS-PAGE gel.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.