BD, 554164, BD Pharmingen™ Purified Mouse Anti-Human Underphosphorylated Retinoblastoma Protein (RB)

Alternative Name: RB (a.a. 514-610)
Reactivity: Human (QC Testing), Mouse (Tested in Development)
Isotype: Mouse IgG1
Immunogen: Recombinant full-length human Rb
Application: Immunoprecipitation (Routinely Tested)
Target Molecular Weight: 105 kDa
Concentration: 0.5 mg/ml
RRID: AB_395279
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Applications include immunoprecipitation at 1-2 µg/one million cells. Rb migrates as multiple, closely-spaced bands between approximately 110-116 kDa when sized on denaturing polyacrylamide gels (i.e. by SDS/PAGE). The multiple bands represent different Rb phosphorylation states, the higher molecular weight bands are more highly phosphorylated than the lower molecular weight bands. The level of phosphorylation is cell cycle dependent, and may also be cell type dependent (not all forms are seen in all cell types that express Rb). Gel conditions influence the actual number of bands observed. In cases where optimal band separation is desired, use a 7 to 10% non-gradient long (at least 12 inches) gel. MOLT-4 human leukemia cells (ATCC CRL-1582) are suggested as a positive control.