BD, 554730, BD Pharmingen™ PE Mouse Anti-Human MIP-1α (CCL3)

Alternative Name: CCL3
Reactivity: Human (QC Testing), Rhesus, Cynomolgus (Tested in Development)
Isotype: Mouse IgG2a, κ
Application: Intracellular staining (flow cytometry) (Routinely Tested), Immunofluorescence (Tested During Development)
Concentration: 0.2 mg/ml
RRID: AB_395532
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures The PE Mouse Anti-Human MIP-1α (CCL3) can be used for multicolor immunofluorescent staining and flow cytometric analyses to identify and enumerate MIP-1α (CCL3) producing cells with mixed cell populations. For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated (≤ 0.5 µg mAb/million cells). A useful control for demonstrating specificity of staining is either of the following: 1) pre-block the conjugated 11A3 antibody with ligand (e.g., recombinant human MIP-1α (CCL3)) prior to staining, or 2) pre-block the fixed/permeabilized cells with unlabeled 11A3 antibody prior to staining. The intracellular staining technique and use of blocknig controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgG2a, κ isotype control immunoglobulin for assessing the level of background staining on paraformaldehyde-fixed/saponin-permabilized human cells is PE Mouse IgG2a, κ Isotype Control (Cat. No. 554648); use at comparable concentrations to antibody of interest (e.g., ≤0.5 µg mAb/1 million cells).