BD, 555038, BD Pharmingen™ Purified Mouse Anti-Human MIG

Alternative Name: C-X-C motif chemokine 9; CMK; crg-10; Humig; SCYB9
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Recombinant Human MIG Protein
Application: ELISA Capture (Routinely Tested), Intracellular block/flow cytometry, Intracellular staining (flow cytometry) (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_395664
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Capture: The purified monoclonal antibody B8-11 is useful as a capture antibody in a sandwich ELISA for measuring human MIG protein levels. Purified monoclonal antibody B8-11 can be paired with the biotinylated monoclonal antibody B8-6 (Cat. No. 555037) with recombinant human MIG (Cat. No. 554636) as the standard. Purified monoclonal antibody B8-11 should be titrated to determine optimal concentration for ELISA capture (0.5-4.0 µg/ml). To obtain linear standard curves, doubling dilutions of human MIG ranging from ~2,500 to 39 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com. This ELISA pair shows no cross-reactivity with any of the cytokines tested ( human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL- 15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, IP-10, lymphotactin, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TNF, LT-α). This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum samples. The BD OptEIA™ ELISA set (Cat. No. 550998) is specially-formulated for cytokine measurement in complex fluids such as serum and plasma. Immunofluorescent Staining and Flow Cytometric Analysis: The B8-11antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify and enumerate MIG producing cells within mixed cell populations. The PE-conjugated B8-11 antibody (Cat. No. 566013) is especially suitable for these studies.