BD, 555049, BD Pharmingen™ PE Mouse Anti-Human IP-10

Reactivity: Human (QC Testing)
Isotype: Mouse IgG2a, κ
Immunogen: Recombinant human IP-10 protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_395670
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The PE-conjugated 6D4/D6/G2 antibody (Cat. No. 555049) can be used for multicolor immunofluorescent staining and flow cytometric analyses to identify and enumerate human IP-10-producing cells within mixed cell populations (See Figure).  For optimal immunofluorescent staining with flow cytometric analysis, this anti-cytokine antibody should be titrated.  (≤ 0.5 µg mAb/1X10^6 cells) For specific methodology, please see the online protocols or the chapter on intracellular staining in the Immune Function handbook posted at www.bdbiosciences.com. A useful control for demonstrating specificity of staining is to pre-block the paraformaldehyde-fixed/saponin-permeabilized cells with unlabeled 6D4/D6/G2 antibody prior to staining. The staining technique and use of blocking controls are described by C. Prussin, et al. A suitable mouse IgG2a isotype control for assessing the level of background staining on paraformaldehyde-fixed/saponin-permeabilized human cells is PE-G155-178 (Cat. No. 554648); use at comparable concentrations to antibody of interest (e.g., ≤ 0.5 µg mAb/1X10^6 cells). ELISA: The biotinylated 6D4/D6/G2 (Cat. No. 555048) antibody is useful as a detection antibody for a sandwich ELISA for specifically measuring human IP-10 protein levels. Biotin 6D4/D6/G2 antibody can be paired with the Purified 4D5/A7/C5 antibody (Cat. No. 555046) as the capture antibody and with Recombinant human IP-10 protein (Cat. No. 551130) as the standard.