BD, 555291, BD Pharmingen™ Purified Hamster Anti-Mouse CD178

Alternative Name: Fas Ligand, FasL; Fas-Lg; CD95 Ligand; CD95L; Tnfsf6; APT1LG1; gld
Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG1, κ
Immunogen: Mouse FasL-transfected cells
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_395709
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures We have found that enriched splenic T cells are induced to express Fas Ligand by 6-8-hour culture with plate-bound anti-mouse CD3 mAb 17A2 (Cat. No. 555273), mAb 145-2C11 (Cat. No. 557306/553058), or mAb 500A2 (Cat. No. 553238). Because Fas Ligand is expressed at low density on activated cells, it may be desirable to amplify staining by using a biotinylated second-step antibody with a "bright" third-step reagent, such as Streptavidin-PE (Cat. No. 554061). The BD Biosciences anti-hamster IgG mAb cocktails, either biotin- or PE-conjugated (Cat. No. 554010 or 554056, respectively) are not effective for staining with mAb MFL3 (see Figure).