BD, 556012, BD Pharmingen™ Purified Rat Anti-Human CD210a

Alternative Name: CD210; CDW210A; IL10RA; IL-10RA; IL-10R-A; IL10R; HIL-10R; IL-10R1; I10R1
Reactivity: Human (QC Testing)
Isotype: Rat F344, also known as Fischer, CDF IgG2a, κ
Immunogen: Human IL-10R alpha Recombinant Protein
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
Workshop Number: IX 30
RRID: AB_396290
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures For flow cytometric applications, a three step labeling procedure is recommended for amplifying signal. Suggested protocol for 3-step staining using Lysed Whole Blood method: 1. Incubate 100µl whole blood with primary (unconjugated) antibody for 20-30 minutes in the dark at room temperature. 2. Add 2 mls of 1X Lysing Buffer (Cat. No. 555899/349202) and incubate for 10-15 minutes in the dark. Centrifuge and aspirate. 3. Wash once with PBS/0.1% sodium azide/1% heat-inactivated fetal bovine serum  (PBS-FBS). Centrifuge and aspirate. 4. Add biotinylated goat anti-rat Ig's and incubate for 20-30 minutes in the dark at room temperature. 5. Wash once with PBS-FBS. Centrifuge and aspirate. 6. Add SAV-PE (Cat. No. 554061) and incubate for 20-30 minutes in the dark at room temperature. 7. Wash once with PBS-FBS. Centrifuge and aspirate. Resuspend in 0.5 ml of PBS-FBS and analyze by flow cytometry.