BD, 557998, BD Pharmingen™ Alexa Fluor® 700 Rat Anti-Mouse IFN-γ

Reactivity: Mouse (QC Testing)
Isotype: Rat IgG1, κ
Immunogen: Mouse IFN-γ Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Target Molecular Weight: 15-17 kDa
Concentration: 0.2 mg/ml
RRID: AB_396979
Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 700 under optimum conditions, and unreacted Alexa Fluor® 700 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures Recommended Assay Procedure: Immunofluorescent Staining and Flow Cytometric Analysis: XMG1.2 is useful for immunofluorescent staining and flow cytometric analysis on fixed and permeabilized cells to identify and enumerate IFN-γ producing cells within mixed cell populations. An appropriate rat IgG1 isotype control is BD Cat. No. 558001. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.