BD, 558609, BD Pharmingen™ Alexa Fluor® 647 Rat anti-Histone H3 (pS28)

Reactivity: Human (QC Testing), Mouse, Rat, Cow, Fly (Reported)
Isotype: Rat IgG2a, κ
Immunogen: Phosphorylated Human Histone H3 Peptide
Application: Bioimaging (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_1645451
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Recommended Assay Procedures: Recommended Assay Procedures 1. Seed the cells in appropriate culture medium at ~10,000 cells per well in a BD Falcon™ 96-well Imaging Plate (Cat. No. 353219), and culture overnight. 2. Remove the culture medium from the wells, and fix the cells by adding 100 µl of fresh 3.7% Formaldehyde in PBS or BD Cytofix™ fixation buffer (Cat. No. 554655) to each well and incubating for 10 minutes at room temperature (RT). 3. Remove the fixative from the wells, and permeabilize the cells using either cold methanol or Triton™ X-100: a. Add 100 µl of -20 ° C 90% methanol or -20 ° C BD™ Phosflow Perm Buffer III (Cat. No. 558050) to each well and incubate for 5 minutes at RT. OR b. Add 100 µl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT. Triton is a trademark of The Dow Chemical Company. 4. Remove the permeabilizer, and wash the wells twice with 100 μl of 1× PBS. 5. Remove the PBS, and block the cells by adding 100 µl of blocking buffer (3% FBS in 1× PBS) or BD Pharmingen™ Stain Buffer (FBS) (Cat. No. 554656) to each well and incubating for 30 minutes at RT. 6. Remove the blocking buffer, dilute the antibody conjugate 1:10 in blocking buffer or Stain Buffer (FBS), and stain the cells by adding 50 µl of the diluted antibody conjugate to each well and incubating for 1 hour at RT. 7. Remove the diluted antibody conjugate, and wash the wells three times with 100 μl of 1× PBS. 8. Remove the PBS, and counter-stain the nuclei by adding 100 μ l of a 2 μ g/ml solution of Hoechst 33342 (eg, Sigma-Aldrich Cat. No. B2261) in 1× PBS to each well at least 15 minutes before imaging. 9. View and analyze the cells on an appropriate imaging instrument.
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). This product is sold under license from Shigei Medical Research Institute, Okayama, Japan. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.